Board Review Corner

Laboratory Diagnosis II

By Jay M. Portnoy, MD

1. Which test is most appropriate for measuring total IgG in a commercial lab?

a) Protein electrophoresis
b) Radial immunodiffusion
c) Nephelometry
d) Radioimmunoassay
e) Western Blot

2. To measure minute amounts of cat allergen in a dust sample, the best test would be:

a) Nephlometry
b) Immunodiffusion
c) Direct immunoassay
d) Inhibition immunoassay
e) Sandwich immunoassay

3. Which is the best test to determine whether donor bone marrow is compatible with a recipient?

a) Mixed Lymphocyte Response
b) Mitogen proliferation study
c) Functional flow cytometry
d) Northern blot
e) Southern blot

4. A reaction is noted 12 hours following an intracutaneous test. What is most likely to be seen on biopsy?

a) Infiltration by eosinophils
b) Infiltration by CD4+ T-cells
c) Infiltration by basophils
d) Infiltration by granulomatous cells
e) Diffuse edema in nonspecific infiltration

5. Which is most likely to augment DTH results:
a) Pregnancy
b) Cimetadine
c) Active tuberculosis
d) Prednisone
e) Lymphoma

Answers:

1. C
2. E
3. A
4. C
5. B